Thawing Sperm of Sheep Towards Increasing Potency of Spermatozoa

The development of cattle population in Indonesia has not reached in happy condition, even in east Java in the 2001 was decreasing population of some cattle, such as sheep 3,24 %, cow 5,86% and buffalo 5% whereas other cattle have increased far from our hope. (Anonymous, 2007). The obstacle which is faced in the cattle breeding field is involving reproduction field, the priority fertilization problem includes failure sperm cell to penetrate zone pellucid on egg cell due to less enzyme acrosin potency and this is the first factor which hampers class cattle reproduction. Focus on the problem mentioned, so the short aim of this research is to get specific identify protein from acrosin that is in the acrosom sheep spermatozoa and to isolate specific protein from acrosin as the bioactive material to make better cattle fertility. As for the further aim of this research is to get supplying specific protein from acrosin as the commercial way as well as to make better cattle fertility by making better its class reproduction. This research is laboratorial experiment, it is done by biological potential test from acrosin with spermatozoa medium without acrosin and spermatozoa + acrosin. The investigation of biological potential test including: motility percent, viability, abnormality, capacity, non-capacity and acrosom reaction. The investigation is divided into 2 incubation times, they are 30 minutes and 60 minutes. The result are: acrosin supplementation 4,5 μgram can increase motility sheep spermatozoa. Acrosin supplementation 3,0 and 4,5 μgram can increase viability, capacity and spermatozoa sheep acrosom reaction EG. The conclusion are: acrosin supplementation (addition) with dosage 4,5 μgram can be used to increase biological sheep EG potency, so it can be used as alternative (bioactive) material to make better cattle sheep fertility.